UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

Autores

DOI:

https://doi.org/10.33362/ries.v7i2.1428

Palavras-chave:

Bactérias patogênicas. Fungos. Ozonização

Resumo

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.

Palavras-chave: Bactérias patogênicas. Fungos. Ozonização.

 

USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES

 

ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.

Keywords: Pathogenic bacteria. Fungi. Ozonization.

Biografia do Autor

Thais Nogueira Gonzaga, Universidade Federal de Uberlândia

Mestre em Ciências Ambientais pela Universidade Brasil

Dora Inés Kozusny-Andreani, Universidade Brasil - Campus Fernandópolis

Professora titular do Mestrado em Ciências Ambientais

Referências

ALAGÖZ, Aylin Zeren; KOCASOY, Günay. Determination of the best appropriate management methods for the health-care wastes in İstanbul. Waste Management, New York, v. 28, 1227-1235, fev. 2008.

APHA-American Public Health Association. Standard methods for the examination of water and wastewater (22nd ed.). Washington, DC: American Public Health Association, 2012.

ASHWORTH, Danielle C.; ELLIOTT, Paul; TOLEDANO, Mirielle B. Waste incineration and adverse birth and neonatal outcomes: A systematic review. Environmental International, London, v. 69, p.120–132, nov. 2014.

BASSEY, Ini U. et al. Environmental and Public Health Aspects of Solid Waste Management at the Lemna Dumpsite in Calabar, Cross River State, Nigeria. International Journal of Tropical Disease & Health, Chicago, v. 10, n.3, p.1-13, jan. 2015, doi: 10.9734/ijtdh/2015/20023

BLENKHARN, Ian. Safe disposal and effective destruction of clinical wastes. Journal of Hospital Infection, London, v. 60, p. 295–297, set. 2005.

BRASIL. Ministério do Meio Ambiente. Conselho Nacional do Meio Ambiente. Resolução n. 358, de 29 de abril de 2005. Dispõe sobre o tratamento e a disposição final dos resíduos dos serviços de saúde e dá outras providências. Brasília, 2005. Disponível em: http://www.mma.gov.br/port/conama/legislacao/CONAMA_RES_CONS_2005_358.pdf

CHAYB, Edilza Felicia; KOZUSNY-ANDREANI, Dora Inés. Estudo comparativo da contaminação por micro-organismos patogênicos em resíduos domiciliares e de saúde em Uberlândia (MG). Revista Brasileira Ciências Ambientais, Rio de Janeiro, v. 37, set. 2015; doi: 10.5327/Z2176-9478201512414

COCCIA, Ana Maria et al. Airborne microorganisms associated with waste managemet al.ent and recovery: biomonitoring methodologies. Annali dell'Istituto Superiore Di Sanita, Roma, v.46, n.3, p.288-292, 2010. doi: 10.4415/ANN_10_03_11.

COKER, Akinwale et al. Medical waste management in Ibadan, Nigeria: Obstacles and prospects. Waste Management, New York, v.29, n.2, p.804–811, fev. 2009. doi.org/10.1016/j.wasman.2008.06.040

GAUTAM, Vidhi; THAPAR, Rajni.; SHARMA Mohita, Resham. Biomedical waste management: Incineration vs. environmental safety. Indian Journal of Medical Microbiology, New Delhi, v.28, p.191–192, jul-set. 2010. http://www.ijmm.org/text.asp?2010/28/3/191/66465

GÜZEL-SEYDIM, Zeynep; BEVER, Paul; GREENE, Annel. Efficacy of ozone to reduce bacterial populations in the presence of food components. Food Microbiology, Estados Unidos, v.21, n.4, p.475-479, aug. 2004. doi.org/10.1016/j.fm.2003.10.001

HAMER, Geoffrey. Solid waste treatment and disposal: effects on public health and environmental safety. Biotechnology Advances, London, v.22, p.71–79, dec. 2003. doi.org/10.1016/j.biotechadv.2003.08.007

HOSSAIN, Sohrab et al. Treatment of Clinical Solid Waste Using a Steam Autoclave as a Possible Alternative Technology to Incineration. International Journal Environmental Research and Public Health, Switzerland, v.9, n.3, p. 855-867;mar. 2012, doi:10.3390/ijerph9030855

KAŹMIERCZUK, Marcin; BOJANOWICZ-BABLOK, Anna. Bioaerosol concentration in the air surrounding municipal solid waste landfill. Environmental Protection and Natural Resource, ,Polonia v.25, n. 2(60), p. 17–25, jun. 2014. doi: 10.2478/oszn-2014-0015

MATTIELLO, Amalia et al. Health effects associated with the disposal of solid waste in landfills and incinerators in populations living in surrounding areas: A systematic review. International Journal of Public Health, Switzerland, v.58, p.725-735, oct. 2013. doi.org/10.1007/s00038-013

MARTINELLI, Mauro et al. Water and air ozone treatment as an alternative sanitizing technology. Journal of Preventive Medicine and Hygiene, Roma, v.58 n.1, , E48-E52, mar. 2017. doi.org/10.15167/2421-4248/jpmh2017.58.1.757

MARTINS, Carmem Costa; KOZUSNY-ANDREANI, Dora Inés; MENDES, Elena Carla Batista. Ozônio no controle de micro-organismos em resíduos de serviços de saúde. Revista Baiana Enfermagem, Salvador, v.29, n.4, p.318-29, out/dez. 2015. doi.org/10.18471/rbe. v29i4.13678.

MURRAY, Byron K. et al. Virion disruption by ozone-mediated reactive oxygen species. Journal of Virological Methods, London, v.153, p.74-77, jul. 2008. doi.org/10.1016/j.jviromet.2008.06.004

NASCIMENTO, Thiago Cesar et al. Ocorrência de bactérias clinicamente relevantes nos resíduos de serviços de saúde em um aterro sanitário brasileiro e perfil de susceptibilidade a antimicrobianos. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v.42, n.4, p.415-419, jul/ago. 2009. doi.org/10.1590/S0037-86822009000400011.

NOGALES, Carlos Goes et al. Comparison of the antimicrobial activity of three different concentrations of aqueous ozone on Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis – in vitro study. Revista Espãnola Ozonoterapia, Madrid, v.4, n.1, p.9-15, 2014.

OLIVEIRA, Emerson A. et al. Microwave inactivation of Bacillus atrophaeus spores in healthcare waste. Waste Management, ,New York, v. 30, p.2327-2335, nov. 2010. doi:10.1016/j.wasman.2010.05.002

ÖKTEN, Hatice Eser; CORUM, Adnan; DEMIR, Hacer Handan. A comparative economic analysis for medical waste treatment options. Environment Protection Engineering,Polonia, v.41, n.3, 2015. doi: 10.5277/epe150310

PARK, Hongrae et al. Detection and hazard assessment of pathogenic microorganisms in medical waste. Journal of environmental science and health, Philadelphia, v. 44, p. 995-1003, ago. 2009. http://www.tandfonline.com/loi/lesa

ROSENBLUM, James et al. Ozonation as a clean technology for fresh produce industry and environment: sanitizer efficiency and wastewater quality. Journal of Applied Microbiology, Oxford, v.113, n.4, p.837-845, mar. 2012. doi: 10.1111/j.1365-2672.2012.05393.x.

RUSSELL, A. Denver. Similarities and differences in the responses of microorganisms to biocides. Journal of Antimicrobial Chemotherapy, London, v.52, n.5, p.750-763, nov. 2003. doi.org/10.1093/jac/dkg422

SAINI, Savita et al.. The study of bacterial flora of different types in hospital waste: Evaluation of waste treatment at aims hospital, New Delhi. Southeast Asian Journal of Tropical Medicine and Public Health, Bangkok, v. 35,n.4, 986-989, dec. 2004.

SHARMA, Manju; HUDSON, James B. Ozone gas is an effective and practical antibacterial agent. American Journal of Infection Control, New York, v.36, p.559-63, oct. 2008. doi.org/10.1016/j.ajic.2007.10.021

SINGH Pragya, Impact of Solid Waste on Human Health: A Case Study of Varanasi City. International Journal of Scientific & Engineering Research, Estados Unidos, v. 4, n.11, p.1840-1842, nov. 2013. http://www.ijser.org

THANOMSUB, Benjamas et al. Effects of ozone treatment on cell growth and ultrastructural changes in bacteria. Journal of Applied Microbiology,Oxford, v.48, p.193-199, jun. 2002.

TORMIN, Stephanie Corradini et al. Análise do efeito bactericida do ozônio sobre bactérias multirressistentes. Arquivos médicos dos Hospitais e da Faculdade de Ciências Médicas da Santa Casa de São Paulo.São Paulo, v.61, p.138-141, 2016.

TORTORA Geralda Jerry, FUNKE Berdell R., CASE Cristiane L. Microbiologia. 10ª. ed. Porto Alegre: Artmed Editora SA; 2012.

TRAVERSI, Deborah et al. Size-fractionated PM10 monitoring in relation to the contribution of endotoxins in different polluted areas. Atmospheric Environmental, Estados Unidos, v.45, p. 3515-3521, jul. 2011. doi.org/10.1016/j.atmosenv.2011.04.020

WANI, Shreya et al. Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach. Agriculture, Switzerland, v.5, p.155-169, jun. 2015. doi:10.3390/agriculture5020155

WINN JUNIOR, Washington. et al. Diagnóstico microbiológico - texto e atlas colorido. 6ª. ed. Rio de Janeiro: Guanabara Koogan; 2008.

ZAR, Jerrold H. Biostatistical Analysis. 5th edition. Essex: Prentice Hall, 2009.

ZHANG, Jian et al. Supercritical carbon dioxide and hydrogen peroxide cause mild changes in spore structures associated with high killing rate of Bacillus anthracis. Journal of Microbiology Methods, Amsterdam, v. 70, p. 442–451, jun. 2007. doi: 10.1016/j.mimet.2007.05.019

Downloads

Publicado

2018-12-01

Como Citar

Gonzaga, T. N., & Kozusny-Andreani, D. I. (2018). UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE. Revista Interdisciplinar De Estudos Em Saúde, 7(2), 125–139. https://doi.org/10.33362/ries.v7i2.1428

Edição

Seção

Estudos Interdisciplinares em Saúde